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normal donkey serum d9663  (Millipore)


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    Structured Review

    Millipore normal donkey serum d9663
    Normal Donkey Serum D9663, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+donkey+serum+d9663/triton+x+100/pm40553926-96-18-22
    Average 90 stars, based on 1 article reviews
    normal donkey serum d9663 - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Amphetamine induces sex-dependent loss of the striatal dopamine transporter in sensitized mice
    Article Snippet: Sections were incubated in PBS containing 1% H2O2 for 15 min, permeabilized 220 with 0.1% Triton X-100 for 1 hr, and preincubated with blocking buffer containing 10% normal 221 donkey serum (D9663, Sigma Millipore), 3% BSA (A2153, Sigma Millipore), 0.1% Triton X-100 222 eN uro Ac ce pte d M an us cri pt 9 (Sigma Millipore) in PBS for 1 h at room temperature.

    Blocking Assay:

    Article Title: Dopamine Transporter Localization in Medial Forebrain Bundle Axons Indicates Its Long-Range Transport Primarily by Membrane Diffusion with a Limited Contribution of Vesicular Traffic on Retromer-Positive Compartments
    Article Snippet: .. Tissue was then washed, permeabilized with 0.1% Triton-X-100 for 1 h, and preincubated with blocking buffer containing 10% normal donkey serum (D9663, Sigma Millipore), 3% BSA (A2153, Sigma Millipore), 0.1% Triton X-100 (Sigma Millipore) in PBS for 1 h at room temperature followed by incubation with primary antibodies at 4°C in PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 3% BSA for 48 h. Primary antibodies were diluted to 1:1000, except for β-IV spectrin (1:400) unless indicated otherwise. ..

    Article Title: TRPC1 channels underlie stretch-modulated sarcoplasmic reticulum calcium leak in cardiomyocytes
    Article Snippet: Cells were then permeabilized for 18 min in 0.25% Triton X-100 (VWR International, Radnor, PA, United States). .. Image-iT Fx Signal Enhancer (I36933, Thermo Fisher Scientific) was applied for 30 min, followed by blocking in 10% normal donkey serum (D9663, Millipore, Billerica, MA, United States) for 60 min. NRVMs were then incubated in primary antibodies for tRFP (AB233, Evrogen) and SERCA2 (MA3-910, Thermo Fisher Scientific) overnight in 2% normal donkey serum at 4°C. .. Secondary antibodies Alexa Fluor 647 (A-31573, Thermo Fisher Scientific) and CF 568 (SAB4600075, Sigma-Aldrich) were applied at room temperature the following morning for 2 h. During imaging, labeled NRVMs were immersed in a solution containing 20 mM MEA, 1% ( v / v ) 2-Mercaptoethanol, and an oxygen scavenging system (glucose oxidase and catalase) in a buffer of 50 mM Tris, 10 mM NaCl, and 10% ( w / v ) glucose.

    Article Title: TRPC1 channels underlie stretch-modulated sarcoplasmic reticulum calcium leak in cardiomyocytes.
    Article Snippet: Cells were then permeabilized for 18 min in 0.25% Frontiers in Physiology frontiersin.org03 Triton X-100 (VWR International, Radnor, PA, United States). .. Image-iT Fx Signal Enhancer (I36933, Thermo Fisher Scientific) was applied for 30 min, followed by blocking in 10% normal donkey serum (D9663, Millipore, Billerica, MA, United States) for 60 min. NRVMswere then incubated in primary antibodies for tRFP (AB233, Evrogen) and SERCA2 (MA3-910, Thermo Fisher Scientific) overnight in 2% normal donkey serum at 4°C. .. Secondary antibodies Alexa Fluor 647 (A-31573, Thermo Fisher Scientific) and CF 568 (SAB4600075, Sigma-Aldrich) were applied at room temperature the following morning for 2 h. During imaging, labeled NRVMs were immersed in a solution containing 20 mM MEA, 1% (v/v) 2-Mercaptoethanol, and an oxygen scavenging system (glucose oxidase and catalase) in a buffer of 50 mM Tris, 10 mM NaCl, and 10% (w/v) glucose.

    Incubation:

    Article Title: Dopamine Transporter Localization in Medial Forebrain Bundle Axons Indicates Its Long-Range Transport Primarily by Membrane Diffusion with a Limited Contribution of Vesicular Traffic on Retromer-Positive Compartments
    Article Snippet: .. Tissue was then washed, permeabilized with 0.1% Triton-X-100 for 1 h, and preincubated with blocking buffer containing 10% normal donkey serum (D9663, Sigma Millipore), 3% BSA (A2153, Sigma Millipore), 0.1% Triton X-100 (Sigma Millipore) in PBS for 1 h at room temperature followed by incubation with primary antibodies at 4°C in PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 3% BSA for 48 h. Primary antibodies were diluted to 1:1000, except for β-IV spectrin (1:400) unless indicated otherwise. ..

    Article Title: Targeting EGLN2/PHD1 protects motor neurons and normalizes the astrocytic interferon response.
    Article Snippet: .. In brief, sections were blocked for 1 h in 10% normal donkey serum (D9663, SigmaAldrich), PBS-T (PBS +0.1% Triton X-100) and incubated subsequently for 1 h at room temperature with the required antibodies (Table S6) dissolved in PBS-T supplemented with 10% normal donkey serum. .. Images were taken with a Leica TCS SP8 confocal laser scanning microscope (Leica Microsystems Heidelberg GmbH, Manheim, Germany) or a Zeiss Axio Scan 7 microsope ((Carl Zeiss Inc.) and analyzed with ImageJ or CellProfiler Image Analysis Software (RRID: SCR_007358).88 Zebrafish Zebrafish were collected at 30 hpf and fixed overnight with 4% PFA at 4 C. Fixation was followed by permeabilization with acetone for 1 h at 20 C, blockingwith 1%bovine serum albumin (BSA), 1%dimethyl sulfoxide (DMSO) PBS for 1 h at RT and immunostaining with primary mouse anti-synaptic vesicle glycoprotein 2 (SV2) (Table S6) in 1%DMSO/PBS for 3 h at RT.

    Article Title: TRPC1 channels underlie stretch-modulated sarcoplasmic reticulum calcium leak in cardiomyocytes
    Article Snippet: Cells were then permeabilized for 18 min in 0.25% Triton X-100 (VWR International, Radnor, PA, United States). .. Image-iT Fx Signal Enhancer (I36933, Thermo Fisher Scientific) was applied for 30 min, followed by blocking in 10% normal donkey serum (D9663, Millipore, Billerica, MA, United States) for 60 min. NRVMs were then incubated in primary antibodies for tRFP (AB233, Evrogen) and SERCA2 (MA3-910, Thermo Fisher Scientific) overnight in 2% normal donkey serum at 4°C. .. Secondary antibodies Alexa Fluor 647 (A-31573, Thermo Fisher Scientific) and CF 568 (SAB4600075, Sigma-Aldrich) were applied at room temperature the following morning for 2 h. During imaging, labeled NRVMs were immersed in a solution containing 20 mM MEA, 1% ( v / v ) 2-Mercaptoethanol, and an oxygen scavenging system (glucose oxidase and catalase) in a buffer of 50 mM Tris, 10 mM NaCl, and 10% ( w / v ) glucose.

    Article Title: TRPC1 channels underlie stretch-modulated sarcoplasmic reticulum calcium leak in cardiomyocytes.
    Article Snippet: Cells were then permeabilized for 18 min in 0.25% Frontiers in Physiology frontiersin.org03 Triton X-100 (VWR International, Radnor, PA, United States). .. Image-iT Fx Signal Enhancer (I36933, Thermo Fisher Scientific) was applied for 30 min, followed by blocking in 10% normal donkey serum (D9663, Millipore, Billerica, MA, United States) for 60 min. NRVMswere then incubated in primary antibodies for tRFP (AB233, Evrogen) and SERCA2 (MA3-910, Thermo Fisher Scientific) overnight in 2% normal donkey serum at 4°C. .. Secondary antibodies Alexa Fluor 647 (A-31573, Thermo Fisher Scientific) and CF 568 (SAB4600075, Sigma-Aldrich) were applied at room temperature the following morning for 2 h. During imaging, labeled NRVMs were immersed in a solution containing 20 mM MEA, 1% (v/v) 2-Mercaptoethanol, and an oxygen scavenging system (glucose oxidase and catalase) in a buffer of 50 mM Tris, 10 mM NaCl, and 10% (w/v) glucose.



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